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Daphnia Magna Toxicity Tests

daphnia magna toxicity testing

The DAPHTOXKIT F magna contains all the materials necessary to perform six acute toxicity tests with the freshwater crustaceans Daphnia magna.

The Daphnia toxicity test has easy to follow instructions and detailed illustrations are provided in the kits for the conduct of range-finding and definitive tests.

The test organisms are included in the kits as dormant eggs (ephippia) which can easily be hatched on demand in 72h to supply the live biota for the daphnia toxicity test.

Product Number TK33 DAPHTOXKIT F
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Resources:

Please review the step-by-step presentation on the DAPHTOXKIT F Procedure:

Daphnia Magna Test Presentation

daphnia magna toxicity testing

Click here to download the Daphnia magna Power Point.  

Comparison Data of MicroBioTests

Microbiotests comparison data daphnia

Click here for comparison data.

Daphnia Toxicity Test Protocol

daphnia toxicity testing

Click here to download the DAPHTOXKIT F short protocol.

Test criterion

The DAPHTOXKIT F microbiotests are 48h assays based on immobility or mortality of the test organisms, with calculation of the EC50 or LC50.

Reproducibility

Ephippia of high quality produced in strictly controlled conditions preclude variability associated with recruitment/maintenance of live stocks in conventional bioassays. 
Highly uniform quality of the test medium is achieved by simple dilution  of  concentrated solutions of  selected salts with de-ionized water.  
Standardized microplate test containers constructed of biologically inert materials ensure uniform exposure conditions. 
A Quality Control Test with a reference chemical is described in detail, for accuracy and reproducibility check.

Cost-Effectiveness

Ephippia can be hatched on demand, eliminating the need and the costs of continuous culturing and maintenance of test organisms.
Minimal equipment needed for test performance: light table or dissecting microscope - small incubator - conventional laboratory glassware.  
Shelf-life of ephippia guaranteed for several months when stored properly, reducing test scheduling constraints.

Contents

Tubes with ephippia, concentrated hatching and toxicant dilution medium, hatching and test containers and micropipettes for the transfer of the organisms. 
Detailed Standard Operational Procedure brochure, abbreviated Bench Protocol, data scoring sheets and graphical L (E) C50 calculation  sheets. 
Specification sheet with batch number of the ephippia and the media.

User-Friendly

Simple handlings and scorings.
Total performance time of the assay approximately 1 hour.
A floppy disc for easy Toxkit data treatment can be obtained on demand.

Sensitivity

Comparable to the sensitivity of Daphnids from laboratory cultures.

Validation

DAPHTOXKITS are used in many laboratories worldwide for routine screening of chemicals and environmental samples. 
Substantial number of publications reporting data for various uses.  
The acute daphnia toxicity tests are strictly adhere to the protocols for regulatory testing with Daphnias prescribed by international organizations such as e.g. the OECD and the ISO.



Optional Components for the DAPHTOXKITF Magna

  • TK33     DAPHTOXKIT F magna with Daphnia Magna (6 tests per kit)
Test Biota
  • TB33     10 Tubes with Daphnia magna ephippia
Test Containers
  • TC32     10 Multi-well plates for Daphnia Magna tests
Test Media
  • TM23     10 Sets of concentrated media for Daphnia magna tests (for 10X2 ISO medium)
Test Food
  • TF14     10 Tubes with Spirulina powder for Daphnia pre-feeding
Test Items
  • TI13     10 Micropipettes for Daphnia tests
  • TI16     10 Microsieves for Daphnia tests
  • TI21     15 Small petri dishes (55mm)
  • TI25     10 Sheets Parafilm for Daphnia magna tests (5 X 4 squares)
Equipment Needed
  • Incubator with lateral illumination
  • Light table with traspartent stage for Daphnia tests
  • Luxmeter
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