Aliivibrio fischeri Toxicity Tests (formerly Vibrio Fischeri )

Toxicity Tests Aliivibrio fischeri



vibrio fischeri toxicity testing kit





Toxicity of environmental samples is an important aspect of pollution monitoring and risk assessment. Commonly employed chemical parameters for analysis are accurate and sensitive for specific molecules under investigation, but give no information regarding biological effects within the ecosystem.

These methods also ignore synergistic effects from compound mixtures and can underestimate toxic potential of a sample. Evaluation of biological effects using rapid, simple and cost effective methods can provide information on whole sample effects and incorporate these important toxicity parameters into regulatory frameworks.

One important method for assessing toxicity quickly and cost effectively is a bioluminescence inhibition assay that employs a marine gram negative bacterium, Aliivibrio fischeri (formerly Vibrio fischeri) as the test species. Light production is directly proportional to the metabolic activity of the bacterial population, and any inhibition of enzymatic activity due to toxicity or cell death causes a corresponding decrease in the bioluminescence produced by the colony. Test samples are exposed to rehydrated bacteria for a pre-determined period and the degree of light emission inhibition is compared to a negative control. In this way, the assay provides a measure of sub-lethal response as well as lethality through the degree of inhibition.

Aliivibrio fischeri Toxicity Tests (formerly Vibrio Fischeri )Kits and Products Available

vibrio fischeri iso toxicity test

Product No. 1243-1000 

The WaterTox STD (Standard) kit is packaged to provide users with a complete setup to replace traditional vibrio fischeri acute toxicity tests (Microtox® users). All required reagents and solutions are included with the kit to permit facile adoption using current detectors like the M500.

 aliivibrio fischeri toxicity test

Product No. 1243-500 

The WaterTox EVO (Evolution) is packaged as a cost effective alternative to the 1243-1000 Water Toxicity Kit. Bacterial reagents are packaged to permit less reagent waste for smaller sample numbers. The operation protocol has also been simplified to reduce pipetting steps and a salt tablet is included instead of OAS solution and sample diluent to reduce costs in shipping. Results can be interpreted by any approved ISO luminometer. 

 aliivibrio fischeri kinetic toxicity test

Product No. 1243-700 

This kit is provides complete solutions for clients requiring a more accurate and robust assay system that employs the kinetic method for determining toxicity. This method accounts for sample coloration and turbidity and can be used for direct measurement of soil and sediment samples without time consuming correction procedures or filtration. Bacterial reagents are packaged to accommodate 1 entire 96-well plate. Cooling block and luminometer manufactured by Labrox is also offered to provide a complete set up. Individual replacement kit components can be purchased and reagent lease options are available for qualified clients.

 Aliivibrio fischeri  Vibrio fischeri contact toxicity test

Product No. 1243-70 

This kit includes reagent, diluent and LumoStix™ devices to conduct 20 individual measurements. Bacteria are packaged to permit 4 determinations per vial and the LumoStix™ devices are manufactures for use in the portable Kikkoman PD30 luminometer. Replacement kit components can be bought individually to meet testing requirements.

 EBPI is currently carrying several preparations of high quality Aliivibrio fischeri test reagents to conduct rapid toxicity assessments in a wide range of environmental samples. The BioTox™ line of reagents and kits are designed to function independently and include all necessary components. The reagents and diluents can also be combined with other commercially available detector systems to produce results. Several different methodologies are available to fit project budget or laboratory setup, and can be used for analyzing various sample sizes, both clean and turbid water, and rapid determination of single tests. The reagents are standardized for use with

ISO 11348-3: Water quality – Determination of the inhibitory effect of water samples on the light emission o

Vibrio Fischeri Toxicity test kit

f Vibrio fischeri (luminescent bacteria test).

ISO 21338:2010 Water quality — Kinetic determination of the inhibitory effects of sediment, other solids and coloured samples on the light emission of Vibrio fischeri (kinetic luminescent bacteria test)

The BioTox™ reagents perform comparably to several similar commercial test systems (Microtox, LumiStox, ToxAlert) when conducted according to the ISO standards mentioned above. The test organisms are extremely sensitive to organic contaminants and show excellent correlation with toxicity assessments performed with other aquatic species like the fathead minnow and Daphnia magna.

The following BioTox™ products are available for purchase according to various methodology

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