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SOS-ChromoTest Kit

 SOS-ChromoTest Kit for Genotoxicty testingSOS-ChromoTest for genotoxicty

 

 

 

 

SOS-ChromoTest TM kit, for rapid detection of genotoxicity or DNA damage.

Product Number 5031 SOS-ChromoTest Kit
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The SOS ChromoTest TM kit is based on a novel genetically engineered E.coli, which measures the primary response of a cell to genetic damage. In just a few hours, the kit provides a clear, completely objective measurement of the genotoxicity of a sample by a simple visual qualitative evaluation of the degree of DNA damage the cell experiences by observing the colour obtained. A more quantitative value may be obtained by spectrophotometry using a micro-plate reader. The SOS ChromoTestTM analyses are highly correlated with the Salmonella 'Ames Test' for certain materials.

Resources:

Please review EBPI's step-by-step presentation on the UMU-ChromoTest Procedure:

SOS-ChromoTest Presentation

SOS Chromotest for genotoxicity

Click here to download EBPI's SOS-ChromoTest Power Point.  

EBPI SOS-ChromoTest Kit Handout

sos chromotest for genotoxicity

Click here to download EBPI's SOS-ChromoTest Kit Handout

SOS-ChromoTest Flow Chart

sos Chromotest kit for genotoxicity

Click here to download EBPI's latest version of the SOS-ChromoTest flow chart

Each kit contains all the reagents and disposable materials necessary to test at least four samples, including dilutions and controls.  

A single SOS ChromoTest TM kit can be used for a total of 96 unit tests. These tests must include standards and controls (using at least 16 units). Thus, a single kit can screen 80 samples for yes/no responses.


 For quantitative measurements, it is often necessary to allow for an adequate range of sample dilutions and replicates, thus testing five to ten samples with each kit.  Below is an example plate used to detect the DNA damage when a genotoxic material is exposed to the SOS ChromoTestTM bacteria.

Potential Applciations

Potential applications of the SOS-ChromoTestTM that take advantage of the genetically engineered strain of E. coli, including testing of effluents for possible mutagenic or carcinogenic genotoxic compounds. It provides for an evaluation of potential genotoxicity, and is a convenient research tool.

SOS-ChromoTest Advantages

  • User-friendly
  • Sensitive
  • Low Cost
  • Reliable
  • Rapid (under four hours)
  • Contains all of the necessary materials to run the test

 

Biotoxicity Products

Please feel free to download the Biotoxicity product handout.  Products include EBPI's line of toxicity testing kits, MicroBioTests line of Toxkits along with information on Microbial Insights line of molecular tools.

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