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Ames Modified ISO Test

Ames Test Modified ISOAmes Modifed ISO Test OECD

 

 

 

Product No: 5041

This Ames test method is a modification of the Ames ISO fluctuation assay. Bacterial strains are pre-exposed to differing concentrations of the mutagenic compounds with a growth media in suspension for 100 minutes. Each dilution is run in triplicate.

The samples are then transferred, mixed with a reversion solution and plated onto a 96-well plate while using a 48-well evaluation format. The samples are incubated for 2-3 days and the number of revertant colonies (individual wells) are counted by colorimetric analysis.

Resources:

Please review EBPI's step-by-step presentations on the Ames ISO Modification Test:

Ames Modified ISO Presentation

modified Ames test OECD 471

Click here to download EBPI`s Ames Modified ISO Power Point.  

EBPI Ames Test Handout

Ames Test information

Click here to download EBPI's Ames Test Handout

Ames Test Modified ISO Flow Chart

Ames Test ISO OECD

Click here to download EBPI's latest version of the Ames Test Modified ISO flow chart

 This method combines the benefits of the ISO method while maintaining the ease-of-use of the Muta-ChromoPlate method. Smaller sample sizes are required and less reagents are used which is similar to the ISO, however, reversion media mixtures amounts and 96-well microplates facilitate pipetting and sample transfer to incubation step which mirrors the Muta-ChromoPlate Ames test procedure. The 48-well format reduces incubation plates required and plate covers reduce loss of highly volatile samples.

This method is a perfect hybrid between facility and efficiency and can be the preferred method depending on sample constituents.

S9 and Activation of Pro-Mutagens (Optional)

 S9 is a crude liver enzyme extract that can, under certain conditions, convert materials without any genotoxic activity to active genotoxic entities. The chemical process involved may be different for different materials. In addition, the lifetime of the activated moieties is extremely variable: some may be extremely short-lived. This is the reason for incubating the S-9 with the bacteria and the tested material at the same time during the assays.

Ames Express Strains - A new alternate for the detection of Pro-Mutagens

EBPI is proud to officially announce the release of our NEW Ames bacterial strains, called the Ames-ExpressTM strains.  Ames-ExpressTM strains are constructed from normal Ames bacterial strains (TA100, TA98, TA1535, TA97a, TA1538), but have been engineered to also express either human P450 or GST-theta liver enzymes internally, which promotes bioactivation of xenobiotic molecules into DNA reactive species in the absence of an S9 mix.   

The Ames Test ISO, Ames Modifed ISO and Muta-ChromoPlate Kit are designed to be run with the traditional Ames Test bacterial strains or EBPI's new Ames Express bacterial Strains.

Biotoxicity Products

Please feel free to download the Biotoxicity product handout.  Products include EBPI's line of toxicity testing kits, MicroBioTests line of Toxkits along with information on Microbial Insights line of molecular tools.

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