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Molecular Testing Kits

 Specializing in Biomolecular Testing Kits for Toxicity, Mutagenicity, and Genotoxicity.

EBPI (Environmental Bio-Detection Products Inc.) is a biotechnology-based company specializing in the manufacture and custom application of simple, rapid, and cost effective methods for detection and monitoring of toxic, mutagenic and genotoxic materials. In addition to being used for evaluation of existing and new chemicals, the kits have found application in assessing environmental pollution risks in drinking water, wastewater, soil and air. We are a research and development-focused laboratory and manufacturing facility continually developing new testing procedures and easy-to-use testing kits for use by research laboratories around the world.

 Ames Test Kits


Ames Test Kit

Product No. 5051

Ames Test Kit

  • Basic Kits contain 1 Ames Bacterial Strain (TA100 Default unless otherwise requested), Reagents, Controls and Plastics (5051)
  • Bacterial Strain Kits contain 2 Ames Bacterial Strains (TA100, TA98 Default unless otherwise requested, Reagents, Controls and Plastics (B5051)
  • S9 Activation Enzymes allow for indirect and direct testing.  (5051S9 and B5051S)
  • Kits can be shipped in 1, 5 or 10 samples formats - Ask EBPI about our OECD 471 Mutagenicity Ames Test Kits.

A microplate version of the traditional "pour plate" Ames Test.  The Muta-ChromoPlateTM Kit is sensitive and user friendly. It is commonly used to detect mutagenic potential of individual chemicals or mixtures of chemicals in water, wastewater, sediment, air aqueous extracts, new chemicals, foods, cosmetics, pharmaceuticals and any other material that can be put into aqueous solution or micro-suspension, such that it can be taken up by the test strain.


 Ames ISO 384 Well TestAmes Test ISO 384 Well Format

Product No. 6041

The Ames ISO Basic Kit is a fluctuation assay verison of the well-known “Ames Test” which uses a 48-well sample exposure format on a 384-well microplate to reduce reagent and plastic used.  The Ames ISO procedure was developed to test mutagenic materials in a variety of sources like environmental air and water samples, foods, personal care products, and industrial effluents by the fluctuation assay which is a variation of the traditional “Ames Test”.

The Ames ISO method provides researchers with a reliable test method which is facile to set up and run, amenable to samples in aqueous solution or suspension and produces an easily-detectable colorimetric endpoint after 2-3 days of incubation at 37 °C.


Ames 96 Well TestModified Ames ISO Test

Product Number 5041

The Ames ISO Basic Kit is a fluctuation assay verison of the well-known “Ames Test” which uses a 48-well sample exposure format on a 384-well microplate to reduce reagent and plastic used. This Ames test method is a modification of the Ames ISO fluctuation assay. Bacterial strains are pre-exposed to differing concentrations of the mutagenic compounds with a growth media in suspension for 100 minutes. Each dilution is run in triplicate.

The samples are then transferred, mixed with a reversion solution and plated onto a 96-well plate while using a 48-well evaluation format. The samples are incubated for 2-3 days and the number of revertant colonies (individual wells) are counted by colorimetric analysis.

 

Genotoxicity Testing Kits

SOS-ChromoTest Biotoxicity TestingSOS ChromoTest Kit

A microplate genotoxicity bioassay based on the primary response of a genetically engineered bacteria to determine DNA genotoxicity damage.  The SOS-ChromoTestTM Kit can be used to evaluate the presence of genotoxic agents in water, wastewater, sediment, air, chemicals, food, cosmetics, and  for any material that can be put into aqueous solution or micro-suspension such that it can be taken up by the test strain.

 

umu-c ISO ISO 13829 test kitumuc test ISO  ISO 13829 (Water Quality- Determination of the genotoxicity of water and waste water using the umu-test

5021 UMU-ChromoTest Kit (Contains all plastics and reagents)

A microplate toxicity bioassay, EBPI has developed the UMU-CHROMOTESTTM into a simple procedure based upon the International Organization for Standardization protocol ISO 13829 (Water Quality- Determination of the genotoxicity of water and waste water using the umu-test), which can be performed easily in a non‑specialized laboratory.

 

Acute Toxicity Testing Kits (E.coli Based Assays)


Acute biotoxicity testing kitAcute Bacterail toxicity test

5032 TOXI-ChromoTest Kit (Contains all plastics and reagents)

A microplate toxicity bioassay to determine acute or chronic toxicity in water, effluents, and other liquids.  The Toxi-ChromoTestTM Kits endpoint is either partial or complete enzyme inhibition related to the toxic stress the test organism is exposed to.  Its endpoint is a simple color change that can be determined visually or using a plate reader at a wavelength of 610nm.


Acute sediment biotoxicity testing kitAcute sediment bio toxicity test

5033 Toxi-ChromoPad (Contains all plastics and reagents)

A pad toxicity bioassay to determine acute toxicity of materials found in sediments, sludge, soils, or other solid waste materials.  The Toxi-ChromoPadTMdoes not rely on the extraction of chemicals from the substrate and thus is a direct measure of biological exposure potential. It is based upon the same test organism and response as the aqueous based Toxi-ChromoTest.

 

Acute Toxicity Testing Kits (Vibrio Fischeri Based Assays)

vibrio fischeri water toxicity test

vibrio fischeri iso toxicity test

Product No. 1243-1000 

The WaterTox STD (Standard) Vibrio Fischeri kit is packaged to provide users with a complete setup to replace traditional vibrio fischeri acute toxicity tests (Microtox® users). All required reagents and solutions are included with the kit to permit facile adoption using current detectors like the M500.

 

vibrio fisheri iso toxicity test

aliivibrio fischeri toxicity test

Product No. 1243-500 

The WaterTox EVO Vibrio Fischeri (Evolution) is packaged as a cost effective alternative to the 1243-1000 Water Toxicity Kit. Bacterial (Vibrio Fischeri) reagents are packaged to permit less reagent waste for smaller sample numbers. The operation protocol has also been simplified to reduce pipetting steps and a salt tablet is included instead of OAS solution and sample diluent to reduce costs in shipping. Results can be interpreted by any approved ISO luminometer. 

 

aliivibrio fischeri kinetic toxicity test vibrio fischeri kinetic toxicity test (Aliivibrio fischeri)

Product No. 1243-700 

This Vibrio Fischeri test kit is provides com

plete solutions for clients requiring a more accurate and robust assay system that employs the kinetic method for determining toxicity. This method accounts for sample coloration and turbidity and can be used for direct measurement of soil and sediment samples without time consuming correction procedures or filtration. Bacterial reagents are packaged to accommodate 1 entire 96-well plate. Cooling block and luminometer manufactured by Labrox is also offered to provide a complete set up. Individual replacement kit components can be purchased and reagent lease options are available for qualified clients.

 

Aliivibrio fischeri  Vibrio fischeri contact toxicity testaliivibrio fischeri contact toxicity test

Product No. 1243-70 

This Vibrio Fischeri contact test kit includes reagent, diluent and LumoStix™ devices to conduct 20 individual measurements. Bacteria (Vibrio Fischeri) are packaged to permit 4 determinations per vial and the LumoStix™ devices are manufactures for use in the portable Kikkoman PD30 luminometer. Replacement kit components can be bought individually to meet testing requirements.


E.coli Test Kits


E coli testing kitquantative e coli testing kit

5061 ColiPlate (Contains 8 tests per kit)

A convenient test for quantitative measure of total coliforms and E. Coli bacteria. The ColiPlate test is designed to meet regulatory guidelines and quantifies density of target bacteria, coliforms and E. Coli, ranging from 5 to 5,000 colony forming-units (cfu) per 100 mL sample, without dilutions.

 

 


Biotoxicity Products

Please feel free to download the Biotoxicity product handout.  Products include EBPI's line of toxicity testing kits, MicroBioTests line of Toxkits along with information on Microbial Insights line of molecular tools.

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